>> Products >> Semen quality analyse system

Risks How should I collect the sample?
Sperm Why should the man be tested first ?
Abnormalities Who needs to have a semen analysis?
AZOOSPERMIA How does the lab analyse the semen ?
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-----Use for human, Horse, cattle, sheep, dog, pig, cat, fish, etc.

Product introduction:
SPY-1005 Semen quality analyse system(Sperm Quality Analyzer, Animal Sperm Analyzers) is magnifies semen samples through microscope, then puts the microscope image into computer through electronic picture pick-up system, and after that carries out detection and analysis of automatic quality-and-quality-determination of the sperm density, activity, motility rate, and characteristics of movement locus.
This system is especially applicable for clinical semen detection and can highly increase the standard of clinical detection. It can be widely used in urological department, and riatrics,

 department of obstetrics and gynecology (test tube baby), birth control and the research of healthy birth and sound care. It can also be applied in some specialized fields, such as biologic research institute, research institute of endangered animal and rare animal, research institute of artificial propagation of livestock, and artificial insemination station, etc. compare with the tradition methods of analysis, this system has a fast speed, a sound quantification, a high accuracy and an abundant detection parameter data, therefore it can provide objective detection foundation for clinic and scientific research. In addition, it has the features as follow:

Being easily operated, causing little pollution and having a high degree of intelligence, ect.

Main Functions:
• Sperm' average speed of curvilinear motion
• Sperm' average point-to-point speed
• Sperm' average speed of route motion
• Velocity distribution of the sperms
• Detecting the total number of the sperms
• Detecting the total number of motile sperms
• Percentage of sperm' density
• Fraction surviving of the sperms
• Side-sway amplitude of sperms
• Sperms' oscillation
• Sperms' forwardness
• Sperms' linear quality
• Whipping frequency of sperms' motion
• Sperms' average parallel-motion angle
• Number of detected sperms
• Percentage of deformity
• Listing of the speed of detected sperms
• Detecting dilution degree
• Detecting viscosity
• Being able to objectively display and print primary motion path graph of sperms
• Being able to automatically mark out the direction of sperm motion
• Being able to independently diagnose and process pathologic images
• Smell
• Color
• PH value
• Being able to independently carry out semen analysis of livestock and endangered animal, and artificial insemination

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Additional tests for a man with an abnormal semen analysis sperm count report
What constitutes a normal seminal analysis? Semen parameters of 243 fertile men

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How to Collect Bull Semen with Animal Semen analysis equipment?
I want to do this! What's This?
It's amazing that any calves born today are the product of live breedings, as there are so many advantages to using artificial insemination. AI breedings aren't limited by geography, so the potential for genetic diversity is increased. Also, a bull owner can collect enough semen from one ejaculate to service hundreds of cows, making it highly profitable. Follow these steps to learn how to collect semen for artificial insemination.

1、Watch experienced handlers collect semen many times before you try it on your own. Notice how the collector steps carefully around the bull to avoid injury.

2、Ask your veterinarian for collection-kit suppliers. Buy several kits so that you have them on hand for shipping fresh semen. These should include extenders and shipping equipment.
3、Make sure the artificial vagina is clean and that the collection bag is securely attached. Fill the artificial vagina with warm water and lubricate it.
4、Allow the bull to mount the teaser cow. Your helper should stay near the front of the animals while you gently direct the bull's penis away from the cow and into the artificial vagina. Take care not to injure the bull, or he may refuse to mate in the future.
5、Tip the artificial vagina gently so that the end with the collection bag is pointing down. When the bull has ejaculated, carefully remove the artificial vagina and allow him to dismount.
6、Time shipping of (extended) fresh semen in proper cooling containers so that the samples arrive within four days. Frozen semen can be stored for years.

How to Collect Horse Semen with Animal Semen analysis equipment?
I want to do this! What's This?
Artificial insemination is widely practiced among horse breeders; in fact, it's allowed by all breed registries except the Thoroughbred. Artificial insemination allows breeders a broader selection of sires, and semen extenders allow one stallion to service many more mares than a live cover. Use the following instructions to learn how to collect semen from your stud horse.

1、Watch many semen collections by experienced handlers before you attempt it on your own. Note carefully how the handlers move around the stallion to prevent injury to him and to themselves. A stallion in the throes of passion is even more dangerous than usual. If he's injured in any way, he may refuse to mate again.
2、Clean the stallion's penis with warm, soapy water and remove the "bean" (an industry term for the smegma that hardens and collects at the tip). Prepare the artificial vagina by filling it with warm water, lubricate and attach the collection bag.
3、Bring the stallion to the area around the phantom mare, then remove him and bring in the mare in estrus. Keep the stallion somewhere that she can see and smell him, but with a barrier so they can't get to each other. Wait until the mare urinates, direct her to the vicinity of the phantom mare. Remove the real mare.
4、Bring the stallion back, allow him to smell the mare's urine and encourage him to go to the phantom mare. This may take some time for a young stallion.
5、Stay close to the stallion and when he has mounted the dummy, direct his penis into the artificial vagina. When the stallion ejaculates, tip the angle of the bag end of the artificial vagina to the ground gently. Feel the lower side of the base of the penis for ejaculatory pulses so you'll know when he's finished. Remove the artificial vagina carefully.
6、Allow the stallion to dismount the dummy on his own.

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Animal Semen analysis equipment
Animal Sperm analyses(Animal Semen analysis) are often incorporated into reproductive toxicity studies in rats. Due to the relative ease of collecting multiple samples throughout a study, semen analysis in non-rodents such as dogs offers the opportunity to assess potential development of functional effects of compounds on male reproduction over time. In the present study, semen parameters were evaluated in beagle dogs during and at termination of a chronic toxicity study with the 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitor, atorvastatin. Male dogs received 0, 10, 40, or 120 mg/kg orally in gelatin capsules for up to 104 weeks (n = 10/group). After 52 weeks of dosing, 3 dogs/group were euthanized, and 2/group were withdrawn from treatment for a 12-week reversal period and euthanized at Week 64. The remaining 5/group continued treatment until Week 104. Semen was collected from all animals for 3 consecutive weeks prior to termination of the 52-week animals (Weeks 50, 51, 52) for analysis of sperm parameters, using manual methods of evaluation. Semen was collected from the remaining animals at Weeks 64, 78, 91, and 104, and was analyzed. At necropsy, testes, epididymides, and prostates were weighed and evaluated histologically, and epididymal sperm counts were determined. Serum cholesterol was decreased 25C60% at all doses during the study. There were no drug-related differences in semen volume and color, total sperm count, and sperm concentration, morphology, progressiveness, and percent motility during treatment with atorvastatin. There were also no effects on reproductive organ weights or histopathology, and no effects on epididymal sperm count. Thus, incorporation of semen analyses into this study allowed the evaluation of potential male reproductive effects in dogs at multiple time points during the study. Statistical power calculations demonstrated acceptable statistical power (> 80%) for semen sperm count, concentration, morphology, and motility with group sizes of 8C10 animals, and for semen sperm count and concentration or epididymal sperm count with group sizes of 3C5 animals, using the methodology described in this paper.
Semen collection.
Semen samples were collected during Weeks 48 and 49 to familiarize the dogs with the collection procedure, and semen was discarded. Since the study was ongoing at the time of the regulatory request, it was not possible to conduct pretest evaluation of semen. The dogs were approximately 2 years old and fully mature at the time semen analyses were incorporated, and semen samples were obtained from all of the dogs during the study. In our experience, semen collection is more successful in beagle dogs that are 1 year old. Optimally, semen analyses can be incorporated into a study prior to its initiation, and pretest semen analyses would be useful to familiarize dogs with the collection procedure and to exclude animals with consistently poor semen quality or uncooperative behavior.

Animal Semen analysis(Animal Sperm analysis) was conducted on all surviving dogs once each week at Weeks 50, 51, 52, 64, 78, 91, and 104. The analyses at Weeks 50C52 were conducted weekly to determine the variability within each animal and to optimize the evaluations at a time when all animals were still on study. The subsequent time points corresponded to the end of the reversal period (Week 64), termination of the study (Week 104), and 2 intervening periods (Weeks 78 and 91) when other toxicity endpoints (e.g., clinical pathology) were already scheduled.

Animal Semen(Animal Sperm analysis) samples were collected by a slight modification of the method described by Seager and Platz (1977). Briefly, semen was collected manually using an artificial vagina (AV) (Nasco Farm and Ranch, Cat. No. C6157N) connected to a graduated 15-mL conical tube. On the specified days, individual males were taken to a separate room where a female dog, preferably in estrus, was held until the male became aroused. The AV, with a small amount of lubricating jelly (Vaseline® or KY® jelly) on the top cuff, was then slipped over the penis and semen was collected. In dogs, the first fraction of semen is a small pre-sperm fraction that is usually relatively clear. A sperm-rich fraction, usually cloudy, and then a prostatic fluid fraction, which can be very large in volume and contains negligible sperm, immediately follow. In this study, the pre-sperm fraction was collected in the same tube with the sperm-rich fraction to avoid sample loss during the time of active thrusting of the dog. When the sperm-rich fraction was obtained (determined by visual determination and the stepping-over behavior of the male), the sample tube was changed. Often the sperm-rich fraction continued into the second collection tube (based on cloudiness of the semen), necessitating changing collection tubes multiple times. The last fraction of prostatic fluid was collected in the tube until the fluid appeared clear and 2C3 ml was collected; then the AV was removed. The semen tubes were placed immediately in a 37<C water bath until motility was assessed, and sperm morphology slides were prepared, generally within 10 min. Semen color was determined in each tube, and semen volume was calculated as the sum of the volumes (to the nearest 0.5 ml) of all sperm-rich fractions.

Allowing the pre-sperm fraction to mix with the sperm-rich fraction during the collection did not appear to affect sperm motility when motility analyses were conducted immediately after each sample collection. However, we have found in subsequent method-development experiments that more complex media, such as DMEM/F12 (Gibco) are needed to maintain sperm motility for longer than 5C10 min, or when samples must be transported from animal rooms to other laboratories.

Animal Semen analysis(Animal Sperm analysis、Animal Semen quality analyse).
Immediately (< 1C2 min) after obtaining the sperm-rich fraction of semen, an aliquot was diluted in 37<C Dulbecco's phosphate buffered saline, pH 7.4 (D-PBS; Gibco) to an appropriate concentration, and loaded immediately onto a 20 µm µCell chamber (Fertility Technologies) on a 37<C warming plate. The chamber was placed on a 37<C stage warmer on an Olympus CH-2 microscope. Live images of sperm were videotaped using a 10x objective, a video camera, a Panasonic WJ-810 time-date generator, and a Panasonic Model AG-1970 videotape recorder. At least 10 unique fields containing approximately 5C25 sperm each were recorded. Later the videotapes were played back by observers blinded to the animal and group numbers. Sperm motility and progressiveness in approximately 7C10 fields were rated visually on a scale of 0C5. The motility scale was: 0, no moving sperm; 1, < 20% motile sperm; 2, > 20% and < 40% motile sperm; 3, > 40% and < 60% motile sperm; 4, > 60% and < 80% motile sperm; 5, > 80% motile sperm. The progressiveness scale was 0, no motility; 1, slight side-to-side movement, no forward progression; 2, rapid side-to-side movement, no forward progression; 3, rapid side-to-side movement, occasional forward progression; 4, slow, steady forward progression; 5, rapid steady forward progression (adapted from Christiansen, 1984).

Sperm morphology slides were prepared by placing a drop of eosin/nigrosin sperm morphology stain on a glass slide. A drop of semen from the sperm-rich sample was placed on the slide near the drop of stain. The semen and stain were mixed on the slide using the long edge of a glass pipette, the mixture was spread in a thin film across the slide and allowed to air dry. A minimum of 200 sperm were evaluated at 100x by technicians blinded to the group and animal numbers. Sperm were classified as normal, abnormal head, abnormal midpiece, abnormal tail, detached head, proximal cytoplasmic droplet, distal cytoplasmic droplet, and bent tail using criteria described previously (Ball et al., 1983; Oettle and Soley, 1988). Analyses were multiparametric: each sperm was categorized for each defect separately.

Since semen volume is the total volume of the sperm-rich fraction (i.e., excluding the prostatic fluid fraction), semen sperm counts were determined in each sample tube, then the total volume of the sperm-rich fraction was calculated. Anmal Semen sperm counts for each tube were determined in 4 replicate counts on a hemocytometer following dilution of semen fractions with D-PBS. Samples were diluted so that the sperm count for each chamber ranged from 15 to 150 sperm whenever possible. The 4 counts were averaged and the total sperm per ejaculate was calculated by adding the sperm counts from all sperm-rich fractions. Sample tubes containing less than 5 million sperm were considered prostatic fluid fractions, and were not included in the semen volume calculation.

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